Patient-Derived Cancer Organoids: Standardized Protocols for Tumor Cell Isolation, Organoid Generation, and Serial Passaging Across Multiple Cancers

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Şahin A., Şener Akçora D., Yılmaz Göler A. M., Çakır M. O., Yılmaz B.

Methods in Molecular Biology, John M. Walker, Editör, Springer Nature, New York, ss.1-29, 2025

• Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
• Basım Tarihi: 2025
• Yayınevi: Springer Nature
• Basıldığı Şehir: New York
• Sayfa Sayıları: ss.1-29
• Editörler: John M. Walker, Editör
• Marmara Üniversitesi Adresli: Evet

Özet

Patient-derived organoids (PDOs) preserve the architecture and heterogeneity of primary tumors and enable clinically relevant ex vivo testing. We describe standardized protocols for tumor tissue dissociation, extracellular-matrix embedding, tumor-type-specific medium formulation, serial passaging, and histologic and genomic evaluation, alongside quality control criteria to ensure viability and reproducibility across major solid cancers. These methods support precision oncology, drug screening, and translational studies using living biobanks of PDOs.