Phase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro

Johnson N. W. , ÖZKAN M. , Burgess A. P. , Prokic E. J. , Wafford K. A. , O'Neill M. J. , ...Daha Fazla

NEUROPHARMACOLOGY, cilt.119, ss.141-156, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier


In vivo, theta (4-7 Hz) and gamma (30-80 Hz) neuronal network oscillations are known to coexist and display phase-amplitude coupling (PAC). However, in vitro, these oscillations have for many years been studied in isolation. Using an improved brain slice preparation technique we have, using co-application of carbachol (10 mu M) and kainic acid (150 nM), elicited simultaneous theta (6.6 +/- 0.1 Hz) and gamma (36.6 +/- 0.4 Hz) oscillations in rodent primary motor cortex (M1). Each oscillation showed greatest power in layer V. Using a variety of time series analyses we detected significant cross-frequency coupling in 74% of slice preparations.