Phase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro

Johnson N. W., ÖZKAN M., Burgess A. P., Prokic E. J., Wafford K. A., O'Neill M. J., ...More

NEUROPHARMACOLOGY, vol.119, pp.141-156, 2017 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 119
  • Publication Date: 2017
  • Doi Number: 10.1016/j.neuropharm.2017.04.009
  • Journal Name: NEUROPHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.141-156
  • Keywords: Neuronal network oscillations, M1, Theta, Gamma, Phase amplitude coupling, PARVALBUMIN-POSITIVE INTERNEURONS, NITRIC-OXIDE SYNTHASE, ENTORHINAL CORTEX, HIPPOCAMPAL-FORMATION, NETWORK OSCILLATIONS, RECEPTOR ACTIVATION, BEHAVING RAT, NEURONAL OSCILLATIONS, CORTICAL OSCILLATIONS, SOMATOSENSORY CORTEX
  • Marmara University Affiliated: Yes

Abstract

In vivo, theta (4-7 Hz) and gamma (30-80 Hz) neuronal network oscillations are known to coexist and display phase-amplitude coupling (PAC). However, in vitro, these oscillations have for many years been studied in isolation. Using an improved brain slice preparation technique we have, using co-application of carbachol (10 mu M) and kainic acid (150 nM), elicited simultaneous theta (6.6 +/- 0.1 Hz) and gamma (36.6 +/- 0.4 Hz) oscillations in rodent primary motor cortex (M1). Each oscillation showed greatest power in layer V. Using a variety of time series analyses we detected significant cross-frequency coupling in 74% of slice preparations.