Endothelin release is augmented with captopril in rat ischemia-reperfusion injury of the liver

Güllüoǧlu B. M. , Aktan A. Ö. , Yeǧen C. , KURTEL H. , Yalin R.

RESEARCH IN EXPERIMENTAL MEDICINE, cilt.196, sa.4, ss.227-233, 1996 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 196 Konu: 4
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1007/bf02576845
  • Sayfa Sayıları: ss.227-233


The reactive oxygen metabolites (ROMs) and the vascular endothelial factors such as endothelins (ETs) and thromboxane A(2) (TxA(2)) were found to be mediators of the reperfusion component of ischemia-reperfusion (I/R) injury. Captopril (CPT), a sulfydryl (-SH) group containing angiotensin-converting enzyme inhibitor, has been shown to reverse I/R injury by its ROM scavenging effect. In this experimental study, the effects of CPT and BM 13.177 (a TxA(2) receptor antagonist) were assesed on liver I/R injury in rats. Four groups of Wistar albino rats were either sham-operated, control, CPT or BM 13.177-treated. The middle and left lateral hepatic arteries and portal veins were occluded in each group but the sham and the corresponding agents were given to the animals prior to I/R injury. After I/R injury, blood was drawn from the suprahepatic inferior vena cava for ET-l-like activity assay and liver tissue samples were obtained for the determination of prostaglandin E(2) (PCE(2)), leukotriene C-4 (LTC(4)) and histopathologic examination, PGE(2) and ET-1 levels were increased significantly in the control group compared with the sham-operated group. In the CPT group, LTC(4), PGE(2) and ET-I levels were significantly increased compared with the control group, while only ET-I levels were not different from those of the control group in the BM 13.177-treated group. It is concluded that ET-1 release increases in response to I/R injury in rat liver and CPT further increases this release. It also appears that CPT has a stimulatory effect on arachidonic acid metabolism in addition to its free radical scavenging effect.