Akademik Veri Yönetim Sistemi
3rd International Eurasian Conference on Biological and Chemical Sciences, Türkiye, 19 Mart 2020
The protective role of milrinone
on skeletal muscle after ischemia-reperfusion of lower extremities
Esin Ak
Marmara University, Faculty of Dentistry, Department of Histology and Embryology,
Istanbul, Turkey,
esinbir @yahoo.com:
Abstract
Ischemia reperfusion injury of skeletal muscle is a commonly
seen complication after vascular surgery. The aim of
this study was to investigate the protective effect of Milrinone (MIL), as a
phosphodiesterase III inhibitor, on skeletal muscle injury after lower
extremity ischemia reperfusion (IR). Twenty one female wistar rats were randomly divided
into 3 groups; Group I (control, n=7): after midline laparotomy, abdominal
aorta was explored. Group II (IR, n=7): normal saline (1cc, intraperitoneally
(ip) was applied before ischemia. Ischemia was induced by clamping the
abdominal aorta for 1 hour (h) and followed by 1 h of reperfusion. Group III
(MIL+ IR, n=7): Milrinone (0.5 mg/kg, ip) was administered before IR. At the
end of experiment, the skeletal muscles of the thigh were taken and
investigated by histological analyses. Approximately 5 μm thick paraffin
sections were stained with Hematoxylin and Eosin (HE) for histologic analyses.
Histopathological damage scoring was performed by following criteria; vasocongestion,
inflammatory cell infiltration and degeneration of the muscle fibers. Each of
the criteria was scored semiquantitatively using a graded scale evaluated as 0
to 3 (0: normal, 1: mild, 2: moderate, 3: severe). Maximum score was calculated
as 9. The average of the damage scores was calculated for each group and
statistical analysis was performed. Control group showed regular morphology with well-organized
muscle fibers. Severe vasocongestion, muscle fibers degeneration and
inflammatory cell infiltration were revealed in the IR group. In MIL+IR, reduced vasocongestion, muscle fibers
degeneration and inflammatory
cell infiltration were observed. Histopathological damage scores of skeletal muscle
samples were found to be significantly higher in IR group than control group.
Treatment by Milrinone before IR significantly reduced the histopathological
damage score. Our histological study revealed
that Milrinone treatment before ischemia may have a protective effect against skeletal
muscle injury caused by IR of lower extremities.
Keywords: Ischemia, Reperfusion, Milrinone, Phosphodiesterase III
inhibitor, Skeletal muscle, Histology.