About 240 natural and man-made gemstone glyptics (artefacts) (seal gemstones and ceremonial stones) and jewelleries with gemstones in the Izmir Archaeological Museum belong to the Anatolian cavitations regarding to the Greco-Persian, Roman, and elder periods. Many of these glyptics are loose gemstone parts, the others were mainly mounted a ring or assembled into various jewellery parts, such as, pendants, necklaces, earrings, and bracelets. These artefacts are identified using non-destructive and non-invasive gemmological investigation methods (hydrostatic balance, refractometre, gemmology microscope, and UV lamb). It is revealed that a total of 108 of these glyptics are collected into the natural gemstone groups. With being revealed the periods and features to be cut and polished of the ancient gemstone artefacts, the dating and estimating the values of the jewelleries with gemstones which are both obtained in the archaeological excavations and come to the museum for giving can be possible. In addition, this study shows that the conservation process of the ancient gemstones and jewelleries with gemstones, when it is not considered the gemmological features of them, results to be a mistake in the evaluation of many objects. (C) 2013 Elsevier Masson SAS. All rights reserved.