The effect of different protease inhibitors on stability of parathyroid hormone, insulin, and prolactin levels under different lag times and storage conditions until analysis

Baykan O., Yaman A., Gerin F., ŞİRİKÇİ Ö., HAKLAR G.

JOURNAL OF CLINICAL LABORATORY ANALYSIS, cilt.31, sa.6, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 6
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1002/jcla.22144
  • Dergi Adı: JOURNAL OF CLINICAL LABORATORY ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: insulin, parathyroid hormone, pre-analytical phase, prolactin, protease inhibitors
  • Marmara Üniversitesi Adresli: Evet

Özet

IntroductionProteolytic cleavage through proteases affects peptide hormone levels, which is of particular significance when the time interval between sampling and analysis is prolonged. We evaluated the stability of parathyroid hormone, insulin, and prolactin molecules (i) with different protease inhibitors such as K(2)EDTA, aprotinin, and protease inhibitor cocktail (PIC), (ii) with different lag times (6-72hours), and (iii) under different storage temperatures (4 degrees C vs room temperature [RT]) until analysis.