Synthesis of 1,4-diazabicyclo[2.2.2]octane and pyridinium based cationic polymers via ROMP technique and examination of their antibacterial activity and cytotoxicity

Kaymaz A. P., Acaroğlu-Degitz İ., Yapaöz M. A., SEZER A. D., Malta S., Aksu B., ...Daha Fazla

Materialia, cilt.5, 2019 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.mtla.2019.100246
  • Dergi Adı: Materialia
  • Derginin Tarandığı İndeksler: Scopus
  • Anahtar Kelimeler: ROMP, DABCO, Pyridinium, Hemolytic concentration, Antimicrobial activity, Cationic polymer, OPENING METATHESIS POLYMERIZATION, ANTIMICROBIAL POLYMERS, CHARGE-DENSITY, SURFACE, MIMICS
  • Marmara Üniversitesi Adresli: Evet

Özet

© 2019 Acta Materialia Inc.This paper focuses on the synthesis of cationic antibacterial polymers that could be a potential source for new-generation antibiotics with well-defined architecture derived from the Ring Opening Metathesis Polymerization (ROMP) technique. Mono- and double-charge bearing quaternary groups have been used to synthesize cationic homopolymers (MWs: 3000 and 10,000 g/mole) and their copolymers (MWs: 5000 g/mole). Hemolytic concentration (HC50, ≥1000 µg/mL) and MTS assay results showed that the polymers are non-toxic. It has been observed that the double-charge bearing polymers have the highest antimicrobial activity (S. aureus= 8 µg/mL) and a high selectivity against S. aureus (>250). Percent killing efficiencies were tested on a glass surface where moderate killing efficiency was observed in the range of 40–80% in 5 min. Cationic charge density and zeta potential studies were used to investigate the mechanisms of antimicrobial efficiency of the polymers in a solution during the action against S. aureus to understand structure-activity relationships. Scanning electron microscopy (SEM) was also conducted for the bacterial morphology assay.