Speciation of nickel and zinc, its short-term inhibitory effect on anammox, and the associated microbial community composition


Kalkan Aktan Ç. , Uzunhasanoglu A. E. , Yapsakli K.

BIORESOURCE TECHNOLOGY, vol.268, pp.558-567, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 268
  • Publication Date: 2018
  • Doi Number: 10.1016/j.biortech.2018.08.011
  • Title of Journal : BIORESOURCE TECHNOLOGY
  • Page Numbers: pp.558-567
  • Keywords: Anammox, Nickel, Zinc, Inhibition, Next generation sequencing, AMMONIUM OXIDATION ANAMMOX, HEAVY-METALS, ACTIVATED-SLUDGE, LANDFILL LEACHATE, NITROGEN REMOVAL, ORGANIC-MATTER, NITRIFICATION, PERFORMANCE, DENITRIFICATION, RECOVERY

Abstract

This study provides insight into the short-term effects of nickel and zinc on anammox. The impacts of these heavy metals are evaluated based on their potentially bioavailable fractions, including the intracellular, surface-bound, soluble, free-ion, and weak (labile) complexes of heavy metals, in the presence of certain inorganic/ organic species. Results showed that the IC50 values for soluble, intracellular, cell-associated, surface-bound, and free-ion Ni concentrations are 5.99, 0.250, 0.930, 0.680, and 1.36 mg/L, respectively. The inhibitory effect of Zn is found to be lower with respect to Ni, with 1050 values of 6.76, 11.9, 15.1, and 2.71 mg/L for the soluble, intracellular, cell-associated, and free-ion Zn concentrations, respectively. This is the first detailed evaluation of anammox inhibition based on the fractionation of heavy metals. Metagenomic analysis reveals that Candidates Kuenenia constitute approximately 89% of the entire Planctomycetes population, whereas Candidates Brocadia are detected in relatively low fractions (3%).