The protective and antioxidant efects of astaxanthine against cisplatin-induced toxicity in rats


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Ada S., Özbeyli D. , Aydoğan S. G. , Yüksel M. , Çekmen M. B.

Turkish Journal Of Biochemistry-Türk Biyokimya Dergisi, vol.43, no.5, pp.16, 2018 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Abstract
  • Volume: 43 Issue: 5
  • Publication Date: 2018
  • Title of Journal : Turkish Journal Of Biochemistry-Türk Biyokimya Dergisi
  • Page Numbers: pp.16

Abstract

OBJECTIVES: Cisplatin (Cis) is a platinum-based drug,which is used as a chemotherapeutic agent.Cis has several side effects such as

ototoxicity,nephrotoxicity and neurotoxicity.Astaxanthin (Ast) is a carotenoid pigment with antioxidant and anti-inflammatory effects.

The aim of this study is to determine the possible protective and antioxidant effects of astaxanthin against cisplatin-induced toxicity in rats

MATERIALS and METHODS: Sprague-Dawley rats were included in the study (n=32).Cis was given i.p. at a dose of 8mg/kg/day for three days.Ast

group received additionally 100mg/kg/day via gavages for 10 days. Control received olive oil at the same dose.Rats were sacrificed,blood was collected

for determination of BUN, creatinine, ALT, AST, hsTNI, TNF-α, IL-6 and liver, kidney and heart tissues were removed for determination of oxidative

stress parameters such as malondialdehyde(MDA),glutathione(GSH) levels and myeloperoxidase (MPO) activity.

RESULTS: The BUN,creatinine, ALT, AST, hsTNI, TNF-α, IL-6 levels are (p<0.001) increased in Cis group to the control group.Cis+ast group reversed

TNF-α, ALT and hsTNI levels. MDA levels were higher in liver and heart tissues in Cis group, because kidney MDA levels were not different. GSH levels in kidney

tissues were different higher in Cis group (p<0.001). MPO activity,which shows inflammation, is increased in all tissues of Cis treated rats.Ast treatment reverses

the effects of Cis via reducing MPO activity,because the antioxidant effects to MDA and-or GSH levels in kidney,heart and liver tissues were not changed.

CONCLUSIONS: Our findings suggested that Cis treatment induced damage in liver,heart and kidney tissues.Inflammation is the major mechanism which can

be reduced with Ast treatment.Results of oxidative stress parameters show that Cis treatment reveals with “Cisplatin resistance” with increasing GSH levels in

these groups