Chemical composition of endemic Stachys subnuda Montbret & Aucher ex Benth. essential oil and its anti-inflammatory and antioxidant activities


ŞEN A. , KÜRKÇÜOĞLU M., BİTİŞ L. , DOĞAN A. , BAŞER K. H. C.

JOURNAL OF ESSENTIAL OIL RESEARCH, vol.31, no.4, pp.326-334, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 4
  • Publication Date: 2019
  • Doi Number: 10.1080/10412905.2019.1567399
  • Title of Journal : JOURNAL OF ESSENTIAL OIL RESEARCH
  • Page Numbers: pp.326-334
  • Keywords: Stachys subnuda, essential oil, anti-inflammatory activity, antioxidant activity, VOLATILE CONSTITUENTS, BETA-CARYOPHYLLENE, PHYTOCHEMICAL ANALYSIS, BIOLOGICAL-ACTIVITIES, ANTICANCER ACTIVITY, DIFFERENT REGIONS, CENTRAL APENNINES, RECH. FIL., L., LAMIACEAE

Abstract

The essential oil of aerial parts of Stachys subnuda Montbret & Aucher ex Benth (S. subnuda) was analyzed by gas chromatography (GC) and GC/mass spectrometry (MS). Anti-inflammatory activity was evaluated by lipoxygenase inhibition assay. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) assays. Sixty-two constituents, comprising 97.1% of the total essential oil composition, were identified. Phytol (25.2%), beta-caryophyllene (11.2%), germacrene D (6.9%), and hexadecanoic acid (6.9%) were the major compounds of aerial part essential oils of S. subnuda. The essential oil showed significant radical scavenging activity with 50% inhibition (IC50) values of 0.124 and 0.204 mg/mL for ABTS and DPPH radicals, respectively. The essential oil exhibited moderate anti-inflammatory activity with an IC50 value of 0.419 mg/mL. The results indicated that S. subnuda essential oil had good anti-inflammatory and antioxidant activity. Also, this is first study on chemical composition, anti-inflammatory and antioxidant activity of S. subnuda essential oil.