Reaction modelling and simulation to assess the integrated use of transketolase and omega-transaminase for the synthesis of an aminotriol

Chen B. H., Sayar A., Kaulmann U., Dalby P. A., Ward J. M., Woodley J. M.

BIOCATALYSIS AND BIOTRANSFORMATION, cilt.24, sa.6, ss.449-457, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 6
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/10242420601068668
  • Dergi Adı: BIOCATALYSIS AND BIOTRANSFORMATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.449-457
  • Anahtar Kelimeler: multistep synthesis, reaction modelling, transketolase, omega-transaminase, one-pot synthesis, ESCHERICHIA-COLI TRANSKETOLASE, CARBON BOND SYNTHESIS, ORGANIC-SYNTHESIS, L-ERYTHRULOSE, SELECTION, ENZYMES, BIOCATALYSIS, OPERATION, CATALYSIS, KINETICS
  • Marmara Üniversitesi Adresli: Hayır

Özet

The most attractive, as well as challenging, multistep organic syntheses would preferably be carried out in a single reactor, as a one-pot synthesis. For biocatalytic syntheses, multistep reactions in one-pot mode bring a number of advantages, while at the same time raising unique challenges such as the compatibility of different biocatalysts. In this paper, we have developed a transketolase-transaminase (TK-TAm) two-step one-pot aminotriol synthesis reaction model, which integrates reaction kinetic models with process characterization (consisting of component degradation as a function of pH and concentration, aldehyde toxicity towards the enzyme, and ketol donor and acceptor side-reactions with TAm). Based on the analysis of the effect of the TAm/TK activity ratio on product yield, simulations provided guidance for further process and biocatalyst development.