Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species


Akyar I., ÇAVUŞOĞLU C., Ayas M., SÜRÜCÜOĞLU S., Ilki A. , Kaya D. E. , ...More

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.48, no.6, pp.1351-1357, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.3906/sag-1801-198
  • Title of Journal : TURKISH JOURNAL OF MEDICAL SCIENCES
  • Page Numbers: pp.1351-1357
  • Keywords: MALDI-TOF MS, identification, Mycobacterium, Mycobacterium tuberculosis complex, nontuberculous mycobacteria, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, SP NOV., SUBSP ABSCESSUS, DISEASE, INFECTION, CULTURES, CHIMERA, RELAPSE, MEMBERS

Abstract

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis.