CYCLOSPORINE-A REDUCES THE SEVERITY OF COLD-RESTRAINT-INDUCED GASTRIC-LESIONS - ROLE OF LEUKOCYTES


COSKUN T., ALICAN I., YEGEN B., SAN T., CETINEL S., KURTEL H.

DIGESTION, cilt.56, sa.3, ss.214-219, 1995 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 3
  • Basım Tarihi: 1995
  • Doi Numarası: 10.1159/000201246
  • Dergi Adı: DIGESTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.214-219
  • Anahtar Kelimeler: CYCLOSPORINE A, MYELOPEROXIDASE, COLD RESTRAINT, POLYMORPHONUCLEAR LEUKOCYTES, MUCOSAL BLOOD-FLOW, RATS, STRESS, ADHESION, INJURY, NEUTROPHILS, ULCERATION, INHIBITION, SUPEROXIDE, VENULES
  • Marmara Üniversitesi Adresli: Evet

Özet

The objective of this study was to determine the role of cyclosporin A (CsA) on cold-restraint-induced gastric lesions. Animals were subjected to 3 h immobilization at 4 degrees C in plastic restraining devices following a starvation period of 48 h. Gastric samples were obtained for the measurement of myeloperoxidase (MPO) activity, an index of number of peroxidase positive cells and thiobarbituric acid-reactive substances (TBARS; lipid peroxidation). Animals were pretreated with CsA which is a potent immunosuppressant and inhibits ischemia/reperfusion-induced polymorphonuclear leukocyte (PMN) infiltration. Cold-restraint administration significantly elevated the tissue MPO activity and TEARS formation. CsA pretreatment significantly reduced the severity of cold-restraint-induced gastric lesions while attenuating the elevated MPO measurements observed during cold-restraint administration. Animals rendered neutropenic with antineutrophil serum (ANS) exhibited significantly less gastric mucosal injury normally observed after cold-restraint stress. Neither CsA nor ANS treatment effected the elevated TEAR levels, indicating that PMNs are not involved in the lipid peroxidation process observed after cold-restraint stress. In conclusion, the results of this study indicate that CsA is capable of inhibiting cold-restraint-induced gastric mucosal injury and can attenuate the cold-restraint-induced increases in gastric MPO measurements. Our results also indicate that PMNs may be the important mediators of cold-restraint-induced gastric lesions.