Assessment of genetic variations of silver lime (Tilia tomentosa Moench.) by RAPD markers in urban and forest ecosystems

FİLİZ E., Birbilener S., ÖZYİĞİT İ. İ., KULAÇ Ş., ORUÇ F. Ç. S.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.29, sa.4, ss.631-636, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1080/13102818.2015.1042049
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.631-636
  • Anahtar Kelimeler: genetic diversity, principal component analysis (PCA), UPGMA, urban ecosystem, AMPLIFIED POLYMORPHIC DNA, ECOLOGICAL CHARACTERISTICS, POPULATION-GENETICS, DIVERSITY, ISTANBUL, DISTRICT, NORTH, CHINA, FLORA
  • Marmara Üniversitesi Adresli: Evet

Özet

In the present study, the genetic diversity analysis of Tilia tomentosa plants was performed by using random amplified polymorphic DNA (RAPD) primers. Twenty eight plant samples, collected from urban (25 members) and forest (3 members) ecosystems, were used in this study. A total of 53 bands were obtained from eight RAPD primers, of which 48 (90.6%) were polymorphic. The percentage of polymorphic loci (P) was found to be 94.29%, the observed number of alleles (Na) was 1.94, the effective number of alleles (Ne) was 1.60, Nei's gene diversity (h) was 0.34 and Shannon's information index (I) was 0.50. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed two major groups. Members of the urban and forest ecosystems showed high genetic similarity (28%-92%) and they did not separate from each other in UPGMA tree. Furthermore, urban and forest genotypes clustered together in principal component analysis.