3D Printing of Gelatine/Alginate/beta-Tricalcium Phosphate Composite Constructs for Bone Tissue Engineering

Kalkandelen C., Ulag S. , Ozbek B., Eroglu G. O. , Ozerkan D., Kuruca S. E. , ...More

CHEMISTRYSELECT, vol.4, no.41, pp.12032-12036, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 41
  • Publication Date: 2019
  • Doi Number: 10.1002/slct.201902878
  • Title of Journal : CHEMISTRYSELECT
  • Page Numbers: pp.12032-12036
  • Keywords: Alginate, Gelatine, beta-tricalcium phosphate, 3D printing, FABRICATION, SCAFFOLDS


Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can provide tissue regeneration with designed porosity and strengthened structure. Current research has concentrated on the fabrication of natural and synthetic polymer-based complex structures that closely mimic biological tissues due to their superior biocompatibility and biodegradabilities. Gelatine/Sodium Alginate hydrogels reinforced with different concentrations of beta-Tricalcium Phosphate (TCP) (10, 13, and 15 wt.%) were studied to form 3D bone tissue. Physical, mechanical, chemical, morphological properties and biodegradability of the constructs were investigated. Furthermore, in vitro biological assay with human osteosarcoma cell line (SAOS-2) was performed to determine the biocompatibility of the constructs. It is found that cell viability rates for all constructs were increased and maximum cell viability rate was attained for 20%Gelatine/2%Alginate/10%TCP (wt.). The present work demonstrates that 3D printed Gelatine/Alginate/TCP constructs with porous structures are potential candidates for bone tissue engineering applications.