Akademik Veri Yönetim Sistemi
uluslararası 6. ilaç kimyası kongresi, Antalya, Türkiye, 22 - 25 Mart 2018, ss.54
Preparation of core-shell type polyacrylate/hydroxyapatite
drug carriers by controlled polymerization
Gamze Koçal, Burcu Oktay and
Nilhan Kayaman Apohan
Marmara
University, Chemistry Department, Faculty of Arts and Sciences, Göztepe,
Istanbul
E-mail: gamzekocall@gmail.com
Synthetic
hydroxyapatite (HA) is a material commonly used in biomaterial engineering and
regenerative medicine for bones [1,2]. Actually, the natural bone composes of
an organic component such as collagen and an inorganic component such as HA.
The inorganic apatite compound offers osteoconductivity, osteoinductivity and a
bone-bonding ability in the process of bone growth or bone healing [3,4]. Biological
apatite is a nanocrystalline multi-substituted carbonated HA, which deficient in
calcium and with a reduced number of structural hydroxyl groups. This
characteristic composition provides apatite with unique physicochemical and
biological properties. HA is particularly useful as a drug carrier since it has
biocompatible, non-toxic and inert in the corrosive body fluid environment. The
porous hydroxyapatite exhibits good physical-chemical properties such as large
surface area, uniform pore size, high pore volume, which allow multiple drug
loading, slow and controlled release.
The goal of this study is to prepare HA based controlled
drug delivery systems with high biocompatibility using the atom transfer
radical polymerization (ATRP). For this reason, we synthesized meso–porous
hydroxyapatite (MHA) by using hydrothermal method in the presence of
cetyltrimethylammonium bromide (CTAB). Since the -OH groups on the surface of
the meso-porous hydroxyapatite are less active and their surface has been
modified with NH2. For this purpose, we used aminopropyltriethoxysilane (APTES). ATPES shows good binding properties to both
organic and inorganic molecules for modification. To obtain HA-based ATRP initiator (MHA-Br), the NH2 groups of
the MHA were then brominated with 2-bromoisobutyryl
bromide. Afterwards, ATRP of amino
methacrylamide (DMAM), fluorescein methacrylate (FMA) and polyethylene glycol monomethacrylate (PEGMA)
monomers were performed on the MHA-Br.
The prepared novel materials were characterized via FT-IR, TGA and DSC. The
morphology of the drug carrier was investigated by STEM and ESEM.
References
[1] S.V.
Dorozhkin, Calcium orthophosphate-based bioceramics, Materials 6 (9) (2013)
3840–3942.
[2] S. Bose,
S. Tarafder, A. Bandyopadhayay, Hydroxyapatite coatings formetallic implants,
in: M. Mucalo (Ed.), Hydroxyapatite (HAp) for Biomedical Applications, Woodhead
Publishing series in Biomaterials,Cambridge, UK, 2015, pp. 143–159.
[3] Chen F,
Tang QL, Zhu YJ, Wang KW, Zhang ML, Zhai WY, Chang J. Hydroxyapatite
nanorods/pol(yvinyl
pyrolidone) composite nanofibers, arrays and three-dimensional fabrics:
electrospun preparation and transformation to hydroxyapatite
nanostructures.Acta Biomaterialia, 2010, 6, 3013-3020.
[4] Bang LT,
Othman R. Aging time and synthesis parameters of nanocrystalline single phase
hydroxyapatite produced by aprecipitation method. Ceramics –Silikáty, 2014, 58,
157-164
Gamze Koçal, Burcu Oktay and
Nilhan Kayaman Apohan
Marmara
University, Chemistry Department, Faculty of Arts and Sciences, Göztepe,
Istanbul
E-mail: gamzekocall@gmail.com
Synthetic
hydroxyapatite (HA) is a material commonly used in biomaterial engineering and
regenerative medicine for bones [1,2]. Actually, the natural bone composes of
an organic component such as collagen and an inorganic component such as HA.
The inorganic apatite compound offers osteoconductivity, osteoinductivity and a
bone-bonding ability in the process of bone growth or bone healing [3,4]. Biological
apatite is a nanocrystalline multi-substituted carbonated HA, which deficient in
calcium and with a reduced number of structural hydroxyl groups. This
characteristic composition provides apatite with unique physicochemical and
biological properties. HA is particularly useful as a drug carrier since it has
biocompatible, non-toxic and inert in the corrosive body fluid environment. The
porous hydroxyapatite exhibits good physical-chemical properties such as large
surface area, uniform pore size, high pore volume, which allow multiple drug
loading, slow and controlled release.
The goal of this study is to prepare HA based controlled
drug delivery systems with high biocompatibility using the atom transfer
radical polymerization (ATRP). For this reason, we synthesized meso–porous
hydroxyapatite (MHA) by using hydrothermal method in the presence of
cetyltrimethylammonium bromide (CTAB). Since the -OH groups on the surface of
the meso-porous hydroxyapatite are less active and their surface has been
modified with NH2. For this purpose, we used aminopropyltriethoxysilane (APTES). ATPES shows good binding properties to both
organic and inorganic molecules for modification. To obtain HA-based ATRP initiator (MHA-Br), the NH2 groups of
the MHA were then brominated with 2-bromoisobutyryl
bromide. Afterwards, ATRP of amino
methacrylamide (DMAM), fluorescein methacrylate (FMA) and polyethylene glycol monomethacrylate (PEGMA)
monomers were performed on the MHA-Br.
The prepared novel materials were characterized via FT-IR, TGA and DSC. The
morphology of the drug carrier was investigated by STEM and ESEM.
References
[1] S.V.
Dorozhkin, Calcium orthophosphate-based bioceramics, Materials 6 (9) (2013)
3840–3942.
[2] S. Bose,
S. Tarafder, A. Bandyopadhayay, Hydroxyapatite coatings formetallic implants,
in: M. Mucalo (Ed.), Hydroxyapatite (HAp) for Biomedical Applications, Woodhead
Publishing series in Biomaterials,Cambridge, UK, 2015, pp. 143–159.
[3] Chen F,
Tang QL, Zhu YJ, Wang KW, Zhang ML, Zhai WY, Chang J. Hydroxyapatite
nanorods/pol(yvinyl
pyrolidone) composite nanofibers, arrays and three-dimensional fabrics:
electrospun preparation and transformation to hydroxyapatite
nanostructures.Acta Biomaterialia, 2010, 6, 3013-3020.
[4] Bang LT,
Othman R. Aging time and synthesis parameters of nanocrystalline single phase
hydroxyapatite produced by aprecipitation method. Ceramics –Silikáty, 2014, 58,
157-164