Akademik Veri Yönetim Sistemi
II. INTERNATIONAL BIOLOGICAL & LIFE SCIENCES CONGRESS BIOLIC 2024, Antalya, Türkiye, 30 Ekim - 02 Kasım 2024, (Yayınlanmadı)
Staphylococcus aureus is a Gram-positive, biofilm-forming bacterium that is a common cause of acne, abscesses, boils and skin infections in humans. S. aureus biofilm is a major problem that causes difficulties in numerous sectors. Although several chemical biofilm destruction techniques are available, these methods pose a significant risk to human health. Therefore, the need and seeking for a natural compound is inevitable. Myrtus communis has been demonstrated to possess various beneficial properties, including antibacterial, antibiofilm and antioxidant effects. In previous studies, it has been reported that M. communis is rich in phenols and flavonoids, which are secondary metabolite contents. This study aims to identify the antimicrobial and antibiofilm activities of M. communis extracts on S. aureus bacteria. Accordingly, dichloromethane and ethanol extracts of M. communis fruits were used and antibacterial and antibiofilm activities were measured spectrophotometrically by serial dilution method in a 96-well microplate. The concentration of biofilm was determined according to the results of antibacterial activity. The experiments were performed in five replicates.As a results of M. communis dichloromethane extract,1200, 600 and 300 µg/ml concentrations were found to have 92.38 ± 0.02, 89.81 ± 0.01 and 78.25 ± 0.02% bacterial inhibition, respectively. In addition, biofilm inhibition of 75 and 37.5 µg/ml concentrations was found to be 69.57 ± 2.76% and 50.86 ± 1.29%, respectively. M. communis ethanol extract didn't show antibacterial activity. However 150, 75 and 37.5 µg/ml concentrations were found to have 85.14 ± 1.34, 58.54 ± 1.88 and 49.04 ± 0.88% biofilm inhibition, respectively. The results show that M. communis fruit extracts have strong antibacterial and antibiofilm properties against S. aureus bacteria. The results showed that two different solvents can observe different results from the same material. Therefore, it is suggested that M. communis plant may be a natural source for the control of S. aureus bacteria.