Comparison of MALDI-TOF MS, nucleic acid hybridization and the MPT64 immunochromatographic test for the identification of M-tuberculosis and non-tuberculosis Mycobacterium species

Samli A., Ilki A.

NEW MICROBIOLOGICA, cilt.39, ss.259-263, 2016 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39
  • Basım Tarihi: 2016
  • Dergi Adı: NEW MICROBIOLOGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.259-263
  • Anahtar Kelimeler: Mycobacteria, MALDI-TOF MS, MPT ICT, Nucleic acid hybridization, DESORPTION IONIZATION-TIME, MASS-SPECTROMETRY, RAPID IDENTIFICATION, CULTURE-MEDIA, DIRECT ASSAY, COMPLEX
  • Marmara Üniversitesi Adresli: Evet

Özet

Mycobacteria are an important cause of morbidity in humans. Rapid and accurate mycobacterial identification is important for improving patient outcomes. However, identification of Mycobacterium species is not easy, due to the slow and fastidious growth of mycobacteria. Recently, biochemical, sequencing, and probing methods have come to be used for identification. This study compared the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of M. tuberculosis and non-tuberculosis Mycobacteria (NTM) to those of nucleic acid hybridization (NAH) and the MPT64 immunochromatographic test.