Synthesis, cytotoxicity, and pro-apoptosis activity of etodolac hydrazide derivatives as anticancer agents

Cikla P., ÖZSAVCI D., BİNGÖL ÖZAKPINAR Ö., ŞENER A., Cevik O., ÖZBAŞ TURAN S., ...More

Archiv der Pharmazie, vol.346, no.5, pp.367-379, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 346 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.1002/ardp.201200449
  • Journal Name: Archiv der Pharmazie
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.367-379
  • Keywords: Apoptosis, Etodolac, Hydrazide-hydrazone, PC-3 prostate cancer cell line, 4-Thiazolidinone, NATIONAL-CANCER-INSTITUTE, ANTIMYCOBACTERIAL ACTIVITY, BIOLOGICAL-ACTIVITIES, POTENTIAL ANTITUMOR, IN-VITRO, DRUG DISCOVERY, CYCLOOXYGENASE-2, PROSTATE, INHIBITOR, CELLS
  • Marmara University Affiliated: Yes

Abstract

Etodolac hydrazide and a novel series of etodolac hydrazide-hydrazones 315 and etodolac 4-thiazolidinones 1626 were synthesized in this study. The structures of the new compounds were determined by spectral (FT-IR, 1H NMR, 13C NMR, HREI-MS) methods. Some selected compounds were determined at one dose toward the full panel of 60 human cancer cell lines by the National Cancer Institute (NCI, Bethesda, USA). 2-(1,8-Diethyl-1,3,4,9-tetrahydropyrano[3,4-b]indole-1-yl)acetic acid[(4-chlorophenyl)methylene]hydrazide 9 demonstrated the most marked effect on the prostate cancer cell line PC-3, with 58.24% growth inhibition at 105M (10 mu M). Using the MTT colorimetric method, compound 9 was evaluated in vitro against the prostate cell line PC-3 and the rat fibroblast cell line L-929, for cell viability and growth inhibition at different doses. Compound 9 exhibited anticancer activity with an IC50 value of 54 mu M (22.842 mu g/mL) against the PC-3 cells and did not display any cytotoxicity toward the L-929 rat fibroblasts, compared to etodolac. In addition, this compound was evaluated for caspase-3 and Bcl-2 activation in the apoptosis pathway, which plays a key role in the treatment of cancer.