Valorisation of shrimp waste via Bacillus marmarensis -obligate alkaliphilic bacterium- for protease production


Yılmaz Serçinoğlu Z. , Pinar O. , Kazan D.

13th International Conference on Protein Stabilization / ProtStab2021 , Plovdiv, Bulgaria, 7 - 09 October 2021, pp.34

  • Publication Type: Conference Paper / Summary Text
  • City: Plovdiv
  • Country: Bulgaria
  • Page Numbers: pp.34

Abstract

Nowadays, we are facing with increasing environmental issues. Replacement of chemical routes with biotechnological routes which use renewable resources for the production of value-added biochemicals is not only sustainable but also crucial to reduce carbon emissions and waste. Extremophilic microorganisms are valuable biocatalysts using different wastes for the production of industrially important biochemicals, such as enzymes and biopolymers (1–3). Crustacean wastes of seafood markets and restaurants have an enormous volume. Shrimp waste is one of those wastes of which annual production reaches up to 6-8 million tons in India and countries of the Far East (4). This amount of waste can be evaluated as a valuable resource for the production of enzymes. In the light of this information, our aim in this study is to evaluate shrimp waste as a nitrogen source for the production of alkaliphilic protease. Bacillus marmarensis is used as the biocatalyst for the valorization of shrimp waste to produce protease. Productions were conducted in duplicate and protease activities were conducted spectrophotometrically as mentioned in literature (5). Firstly, different carbon sources supplementing basal medium were tested to find the highest protease activity. Then nitrogen source is replaced with different concentrations of shrimp waste to find the optimum condition with the highest protease activity. Our results showed that sucrose is the optimum carbon source and 3% is the optimum shrimp waste concentration.

Our results are promising in terms of circular economy perspective. Not just shrimp, but also other agro-industrial wastes are cheap for sustainable production of valuable biochemicals.