Neonatal BCG vaccination induces IL-10 production by CD4+CD25+T cells

AKKOÇ T. , Aydogan M., Yildiz A., Karakoc-Aydiner E. , Eifan A., Keles S., ...More

PEDIATRIC ALLERGY AND IMMUNOLOGY, vol.21, no.7, pp.1059-1063, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 7
  • Publication Date: 2010
  • Doi Number: 10.1111/j.1399-3038.2010.01051.x
  • Page Numbers: pp.1059-1063
  • Keywords: Bacillus Calmette-Guerin, IL-10, IFN-gamma, children, purified protein derivative, MYCOBACTERIUM-TUBERCULOSIS INFECTION, CALMETTE-GUERIN-VACCINATION, BIRTH


To determine the optimal time of Bacillus Calmette-Guerin (BCG) vaccination for induction of Th1 immunity, we measured the interferon (IFN)-gamma and interleukin (IL)-10 secretion in purified protein derivative (PPD)-stimulated peripheral blood mononuclear cell (PBMC) cultures in newborns vaccinated at birth or 2nd month of life. Moreover, role of CD4+ CD25+ T cells was studied by depletion assay at 8th month. Nineteen term and healthy newborns were randomized into two groups: Group I composed of 10 newborns vaccinated with BCG at birth and the remaining 9 (group II) at 2nd month of life. PBMCs were isolated at birth, 2nd and 8th months of age, and PPD-stimulated IL-10, 5 and IFN-gamma secretion were assessed. The same measurements were repeated for IL-10 and IFN-gamma after the depletion of CD4+ CD25+ T cells at the 8th month. Children vaccinated at birth demonstrated higher PPD-stimulated IFN-gamma and IL-10 levels at 2 months of age when compared to non-vaccinated ones (p = 0.038 and p = 0.022, respectively), whereas at 8 months, no significant differences were detected between the two groups. Moreover, CD4+ CD25+-depleted T-cell cultures resulted in lower PPD-stimulated IL-10 levels in those vaccinated at birth when compared to non-depleted condition at the 8th month (p < 0.001). BCG at birth upregulated PPD-stimulated IFN-gamma secretion at the 2nd month and remained still detectable at 8 month after the vaccination, whereas those vaccinated at the 2nd month of life lacked that increase in IFN-gamma response at the same time-point. Furthermore, depletion assays suggest that CD4+ CD25+ T cells are involved in PPD-stimulated IL-10 secretion in response to BCG vaccination.