Canine filariosis around Istanbul, Turkey employing naphtol AS-TR phosphatase


Toparlak M., Gargili A. , Esatgil M., Cetinkaya H.

ACTA VETERINARIA BRNO, vol.74, no.2, pp.233-237, 2005 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 2
  • Publication Date: 2005
  • Doi Number: 10.2754/avb200574020233
  • Title of Journal : ACTA VETERINARIA BRNO
  • Page Numbers: pp.233-237
  • Keywords: dog, filaria, Dipetalonema recondition, Knott-technique, naphtol AS-TR-phosphatase, DIPETALONEMA-RECONDITUM, DIROFILARIA-IMMITIS, MICROFILARIAE, DIFFERENTIATION, IDENTIFICATION

Abstract

The aim of this study was to find the prevalence of filarial nematodes in dogs in Istanbul, Turkey and identify the species by using naphtol AS-TR phosphatase technique. A total of 286 blood samples were taken from the dogs of different areas of Istanbul, Turkey, between March 1999 and November 2002. The Knott technique was used to detect the microfilariae in blood. Two dogs were found to be positive for microfilariae. The positive blood samples were stained by naphtol AS-TR phosphatase for differentiation. The microfilariae were identified as Dipetalonema recondition. In this study, differentiation of canine microfilaria based on somatic distribution of acid phosphatase activity was performed for the first time and according to these results D. reconditum was reported for the first time from Turkey.