Aqueous electrospun core/shell nanofibers of PVA/microbial transglutaminase cross-linked gelatin composite scaffolds

Sengor M., Ozgun A., Gündüz O., Altintas S.

MATERIALS LETTERS, cilt.263, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 263
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.matlet.2019.127233
  • Dergi Adı: MATERIALS LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Core shell nanofiber, Microbial transglutaminase, Aqueous electrospun, Green electrospinning, Biomaterials, Nanocomposites
  • Marmara Üniversitesi Adresli: Evet

Özet

This study aims to produce gelatin-containing nanofibers in situ cross-linked with microbial transglutaminase (mTG) without using toxic, expensive small-molecule cross-linkers or solvents. Dynamics of enzymatic cross-linking activity was assessed with different enzyme concentrations. Possible time intervals for electrospinning of gelatin-mTG solutions were determined. Gelatin-mTG solution was electrospun as a shell on polyvinyl alcohol core nanofibers and the mTG concentration was optimized to obtain uniform, bead-free nanofiber morphology. For gelatin-mTG solutions, viscosity values below similar to 9.5 centipoise (cP) allowed electrospinning. An mTG concentration of 0.5% (wt/wt) sustained viscosity levels that allow electrospinning for 240 min. Cross-linking activity of mTG was determined by physical and chemical characterization of obtained nanofibers. (C) 2019 Elsevier B.V. All rights reserved.