Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5


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Gullu G. , Peker I., Haholu A., EREN F. , Kucukodaci Z., Gulec B., ...More

GENETICS AND MOLECULAR BIOLOGY, vol.38, no.1, pp.21-29, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1590/s1415-475738120140167
  • Title of Journal : GENETICS AND MOLECULAR BIOLOGY
  • Page Numbers: pp.21-29
  • Keywords: breast cancer, ER alpha, IGFBP5, micro RNA, miR-140, miR-193b, FACTOR-BINDING PROTEIN-5, REPRESSES CELL-PROLIFERATION, ESTROGEN-RECEPTOR-ALPHA, GROWTH, MICRORNAS, TUMORIGENESIS, METASTASIS, SUPPRESSES, MIGRATION, INVASION

Abstract

The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased miR-140-5p expression (p = 0.009). miR-140-5p expression was elevated in invasive ductal carcinomas (p = 0.002), whereas basal-like tumors had decreased expression of miR-140-5p compared to other tumors (p = 0.008). Lymph node-positive samples showed an approximately 13-fold increase in miR-140-5p expression compared to lymph node-negative tissue (p = 0.049). These findings suggest that miR-140-5p, but not miR-193b, could be an important determinant of IGFBP5 expression and clinical phenotype in breast cancer patients. Further studies are needed to clarify the expressional regulation of IGFBP5 by miR-140-5p.