Chemical composition and biological activities of endemicTripleurospermum conoclinium(Boiss. & Balansa) Hayek essential oils


Servi H., ŞEN A. , DOĞAN A.

FLAVOUR AND FRAGRANCE JOURNAL, vol.35, no.6, pp.713-721, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 6
  • Publication Date: 2020
  • Doi Number: 10.1002/ffj.3610
  • Title of Journal : FLAVOUR AND FRAGRANCE JOURNAL
  • Page Numbers: pp.713-721
  • Keywords: antidiabetic activity, anti-inflammatory activity, antioxidant activity, essential oil, Tripleurospermum conoclinium, TRIPLEUROSPERMUM DISCIFORME EXTRACT, ANTIOXIDANT ACTIVITY, BETA-CARYOPHYLLENE, FLAVONOIDS, ASTERACEAE, PLANTS

Abstract

The volatile oils of flowers (TFE), leaves (TLE) and stems (TSE) ofTripleurospermum conoclinium(Boiss. & Balansa) Hayek were obtained by hydrodistillation method for 3 hours with Clevenger-type apparatus. Chemical composition of oils was determined by GC-MS analyses. Antidiabetic, anti-inflammatory and antioxidant activities of oils were tested by alpha-glucosidase inhibitory, 5-lipoxygenase inhibitory and DPPH/ABTS radical scavenging methods, respectively. Eighty-five constituents were identified in flower oil (78.3%). The major compounds of flower oil were artemisia ketone (10.5%), beta-oplopenone (6.0%), tricosane (4.5%), terpinen-4-ol (3.7%) and artemisia alcohol (3.5%). Sixty-two constituents were identified in leaf oil (67.0%). 1-octadecanol (11.5%), caryophyllene oxide (5.7%), elemol (3.9%), phytol (3.5%), ledene oxide (3.3%) and artemisia ketone (3.1%) were found as main constituents in the leaf oil. Forty-six constituents were determined in the stem oil (75.1%). The main compounds of the stem oil were 1-octadecanol (11.1%), beta-oplopenone (5.5%), ledene oxide (5.5%), caryophyllene oxide (4.4%) and phytol (3.0%). TLE and TSE displayed strong antidiabetic activity with IC(50)values of 21.30 and 29.57 mu g/mL against alpha-glucosidase enzyme, respectively. TLE showed the best anti-inflammatory activity with an IC(50)value of 91.02 mu g/mL while TFE and TSE exhibited moderate activity with IC(50)values of 186.50 and 257.10 mu g/mL, respectively. TFE and TLE were found to possess moderate antioxidant activity with IC(50)values of 572.80 and 385.80 mu g/mL against ABTS radical while all essential oils showed weak activity against DPPH radical. To the best of our knowledge, this is first report on chemical composition and biological activities ofTconocliniumessential oils.