Effects of N-acetylcysteine on oxidant-antioxidant balance in oleic acid-induced lung injury


Koksel O., Ozdulger A., Ercil M., Tamer L., Ercan B., Atik U., ...Daha Fazla

EXPERIMENTAL LUNG RESEARCH, cilt.30, sa.6, ss.431-446, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 6
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1080/01902140490476319
  • Dergi Adı: EXPERIMENTAL LUNG RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.431-446
  • Anahtar Kelimeler: acute lung injury, N-acetylcysteine, Na plus -K plus ATPase, oleic acid, total antioxidant capacity, PULMONARY VASCULAR-PERMEABILITY, ISCHEMIA-REPERFUSION INJURY, SPINAL-CORD-INJURY, LIPID-PEROXIDATION, LIQUID VENTILATION, OXIDATIVE STRESS, RAT IRIS, GLUTATHIONE, SUPEROXIDE, DAMAGE
  • Marmara Üniversitesi Adresli: Hayır

Özet

The antioxidant and anti-inflammatory properties of N-acetylcysteine has been documented in. many experimental lung injury models. Because intravenous injection of oleic acid induces histopathologic changes similar to those seen in human acute lung injury or acute respiratory distress syndrome, the authors evaluated the effects of N-acetylcysteine (NAC) on oxidative stress and lung damage in. an oleic acid, (OA)-induced lung injury model. Thirty-five rats were divided. into 5 groups as sham, NAC, OA, pre-OA-NAC, and post-OA-NAC. Lung damage was induced by intravenous administration of oleic acid. Pre-OA-NAC group received intravenous (IV) N-acetylcysteine 15 minutes before oleic acid infusion and post OA-NAC group received IV N-acetylcysteine 2 hours after oleic acid infusion. In both of the N-acetylcysteine treatment groups, blood and tissue samples were collected 4 hours after oleic acid infusion, independent from the time of N-acetylcysteine infusion. In other groups, blood and tissue samples were collected 4 hours after ethanol, NAC, or OA infusions. Serum myeloperoxidase activity, total antioxidant capacity, malondialdehyde levels, and lung tissue Na+-K+ ATPase activity were measured and light microscopic analyses of lung specimens were performed. The administration of N-acetylcysteine significantly restored Na+-K+ ATPase activity and total antioxidant capacity levels and ameliorated lung architecture. N-acetylcysteine has been shown to have some attenuating effects in experimental animal studies. However, further investigations are necessary to suggest N-acetylcysteine as a treatment agent in critically ill patients with lung injury.