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Turk Anesteziyoloji ve Reanimasyon, vol.30, no.5, pp.198-202, 2002 (Scopus)
In our experimental study, effects of poly (ADP-ribose) synthetase (PARS) inhibition on diaphragm Ca++-ATPase, malondialdehyde (MDA) levels and histopathology in LPS-induced sepsis are investigated. 24 male Wistar rats, weighing 180-200 g were randomly divided into 4 groups. The first group (control; n=6) received saline and II. group (sepsis; n=6) received 10mg/kg LPS intraperitoneally. As a PARS inhibitor; 3-Aminobenzamide (3-AB) was given to the IV. group (S+3-AB, n=6) 20 minutes before giving LPS and to the III. group (C+3-AB, n=6) 20 minutes before giving saline. Six hours later, under Ketamin/Xylasine anaesthesia diapraghmatic specimens were obtained and the rats were decapitated. Tissue MDA levels and Ca++-ATPase activity were measured and diaphragmatic specimens were stained with hematoxylin-eosin for histopathologic assesment. Tissue Ca++-ATPase levels were found to be decreased (548±14 vs 215±22 nmol.Pi/mg.protein/hr) and tissue MDA levels were found to be increased (4.2±0.1 vs 11.7±0.4) in endotoxemic group(p=0.01). In the S+ 3-AB group, 3-AB pretreatment attenuated the increase in MDA formation and Ca++-ATPase levels were similar to those in control group (p=0.01). Except minimal edema, no difference, was seen in diaphragm histopathology among the groups. PARS inhibition with 3-AB prevents not only lipid peroxidation but also biochemical changes concerning Ca++-ATPase in endotoxemia.