The marine species are specially used for the fabrication of bioceramic nano-powders with natural methods for their use in controlled drug delivery. However, there are only very limited studies regarding the production and synthesis of hydroxyapatite (HA)-based drug delivery systems from marine structures. In this study, poly (vinyl alcohol) (PVA) containing Rifampicin (RIF)-loaded Orange Spiny Oyster Seashell (Spondylus barbatus) hydroxyapatite (HA) composite is synthesized by an in situ ultrasound-assisted method. All samples were analyzed by X-ray diffraction (XRD), Fourier transforms infrared (FTIR) spectroscopy, and Scanning electron microscope (SEM), respectively. The in vitro drug release tests of the obtained samples were performed in a phosphate-buffered medium (PBS) at 37 degrees C. Drug release was evaluated according to five varying kinetic models. In vitro RIF release from HA/PVA composite in phosphate buffer (pH 7.4) showed prolonged sustained drug release. From the drug release kinetic models, Higuchi and Korsmeyer-Peppas were found to be the best model for the three ratios based on the correlation coefficient. The diffusion component is less than 0.5, which indicates quasi-fickian diffusion. From the kinetic study results, the RIF-loaded marine phase composite has potential use in drug delivery applications as it shows positive sustained drug release behavior.