Oleic acid-induced lung injury in rats and effects of caffeic acid phenethyl ester


KÖKSEL M. O., KAPLAN M. B., ÖZDÜLGER A., TAMER L., değirmenci , CİNEL Z. L., ...Daha Fazla

EXPERIMENTAL LUNG RESEARCH, cilt.31, sa.5, ss.483-496, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1080/01902140590918876
  • Dergi Adı: EXPERIMENTAL LUNG RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.483-496
  • Anahtar Kelimeler: acute lung injury, caffeic acid phenethyl ester, Na(+)-K(+) ATPase, oleic acid, RESPIRATORY-DISTRESS-SYNDROME, ISCHEMIA-REPERFUSION INJURY, SPINAL-CORD-INJURY, NITRIC-OXIDE, LIPID-PEROXIDATION, OXIDATIVE STRESS, ALPHA-TOCOPHEROL, PROPOLIS, SUPEROXIDE, EXPRESSION
  • Marmara Üniversitesi Adresli: Hayır

Özet

Caffeic acid phenethyl ester (CAPE) is a phenolic antioxidant and is an active antiinflammatory component of honeybee propolis. The authors evaluated the effects of CAPE on oxidative stress and lung damage in an oleic acid (OA)-induced lung-injury model. Rats were divided into 5 groups as sham, OA, CAPE, pre-OA-CAPE, and post-OA-CAPE. Acute lung injury was induced by intravenous administration of 100 mg/kg of OA. Pre-OA-CAPE group received CAPE (10 mu mol/kg. intravenously) 15 minutes before OA infusion and post-OA-CAPE group received CAPE 2 hours after OA administration. Malondialdehyde (MDA) level of plasma, bronchoalveolar lavage fluid (BALF), and lung tissue; myeloperoxidase activity of BALF and lung tissue; Na(+)-K(+) ATPase activity of lung tissue; and total protein content of BALF were measured. Light microscopic analyses of lung specimens were performed. The increased AMA levels in lung homogenates (47.98 +/- 13.75 nmol/mL), BALF (31.12 +/- 3.07 nmol/mL), and plasma (61.84 +/- 15.34 nmol/mL) decreased significantly to 24.33 +/- 3.09 nmol/mL (P = 0.000), 23.19 +/- 4.97 nmol/mL (P= 0.002), and 27.36 +/- 5.37 nmol/mL (P= 0.000), respectively, following CAPE administration in pre-OA-CAPE group. Another important finding was the restoration of the enzymatic activity of Na(+)-K(+) ATPase from a value of 203.89 +/- 32.18 nmol Pi/mg Protein/h in OA group, to a value of 302.17 +/- 51.90 nmol Pi/mg Protein/h (P = 0.012) in pre-OA-CAPE group with CAPE treatment. CAPE has been shown to have a clear attenuating effect on oxidative damage in experimental animal studies. However, further investigations are necessary to suggest CAPE as a treatment agent in critically ill patients with lung injury.