Previous studies have identified that genetic variation is a significant determinant of physical activity-related behaviors and for sport enthusiasm. Dopamine receptor genes have been linked to a higher degree of overall physical activity. The dopamine receptor D2 (DRD2) rs1800497 polymorphism affects the brain’s dopaminergic system and athletic performance. However, little research has been done on the connection between DRD2 and sports participation, and the few studies that have been published are contradictory. This study aimed to determine the genotype and allele distributions of the DRD2 rs1800497 polymorphism in football players and compare them with the control group. For this purpose, 21 football players and 52 participants for control group were enrolled in our study. Genotyping was performed by PCR after DNAs were isolated from buccal epithelial cells. When we examined the genotype distributions, the AG and GG were found as 5 (24%) and 16 (76%), respectively. No AA genotype was found for DRD2. When allelic distributions were examined in the athlete group, the A allele was counted as 5 (12%) and the G allele as 37 (88%). In the control group, allelic distributions for A allele and G allele were 36 (%35), 68 (%65), in respectively. As we expected, in our study cohort, the DRD2 rs1800497 polymorphism was found to be dominated by the GG genotype and the G allele. However, we found no statistically significant differences between athlete and control group. Despite the limited sample size, the findings of this study may serve as a foundation for a larger study and point the way for future studies. Therefore, further studies with athletes from different branches and with larger study group are needed to reveal the effect of DRD2 rs1800497 polymorphism on athletic performance and physical activity-related behaviours.