Altuntaş E., Kılıç S. S., Tornacı S., Yılmaz Atalı P., Tuncer S., Akkoç T., ...Daha Fazla
25. Uluslarası Restoratif Diş Hekimliği Derneği Kongresi, Kasım 2024
-
Ödülün Kapsamı:
Diğer Ödüller
-
Ödül Türü:
Diğer
-
Ödül Veren Ülke:
Türkiye
-
Ödülü Veren Organizasyon:
25. Uluslarası Restoratif Diş Hekimliği Derneği Kongresi
-
Araştırma Alanları:
Sağlık Bilimleri
-
Ödülün Tarihi:
Kasım 2024
-
Açıklama:
<p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">Evaluation of Viability Tests of Levan-Based Hydrogels Developed for Use in Dentin Pulp </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">Complex Regeneration by Tissue Engineering Approach on Dental Pulp Derived </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">Mesenchymal Stem Cells </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;"><br></p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">AIM: The current limitations of vital pulp treatments, in terms of the materials used, have prompted efforts to develop new materials that will facilitate regeneration through tissue engineering applications. The objective of this study was to assess the viability of levanbased hydrogels with anti-inflammatory and anticoagulant properties and mineral trioxide aggregate (MTA) and tricalcium silicate-based cement (Biodentin) utilized in current treatments, on dental pulp-derived mesenchymal cells (DPSCs). </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;"><br></p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">METHOD: Enzymatic levan (EL) was produced using levansucrose enzyme obtained from recombinant Halomonas smyrnensis AAD6T, and low molecular weight hydrolyzed levan (EhL) was obtained by microwave acid hydrolysis method. EhL was employed in the </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">production of hydrogel with 1,4-butanediol diglycidyl ether (BDDE) as a cross-linking agent at a temperature of 50°C. Second hydrogel-formulation was created by combining nanohydroxyapatite (nHAP) with EhL(1:1 ratio). MTA and Biodentin samples were prepared </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">using 8-mm diameter silicone disks (Levan+nHAP; Levan, Biodentin, MTA, and DPSCs) were prepared. All prepared samples were sterilized under UV-light and then maintained in a complex medium (C-DMEM) at 37.5°C for 3-days. DPSCs were stored at -80°C, characterized using a FACS Calibur flow-cytometry device. DPSCs were enumerated via Trypan Blue staining following centrifugation and were subsequently seeded in 96-well plates at a density of 100,000 cells per plate. The extracts prepared with sterile agents in the experimental groups were applied to the cells, and viability tests were conducted after 24h, 48h, 120h. Viability and mitochondrial activity were assessed through the MTT assay, and the results were analyzed using a one-way ANOVA test with a significance level of p < 0.05. </p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;"><br></p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">RESULTS: The levan-based hydrogels demonstrated a notable enhancement in cell viability at the 48h (164.2-123.4). However, a considerable decline was observed at the 120h interval (73.4-22.0). In contrast, the viability rates observed in the MTA demonstrated a continuous increase until 120h, with a mean value of 21.8. Levan hydrogel and Levan hydrogel and MTA exhibited statistically significant differences compared to the positive control group (p<0.05). However, no significant difference was observed for the Biodentine (p = 0.333). Levan hydrogel and MTA groups exhibited notable differences, particularly at 48 and 120h. </p>
<p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;"><br></p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">CONCLUSION: The findings indicate that levan-based hydrogels have a favorable impact on cell viability in the short term. However, MTA may offer a more sustained effect over the long term. Levan-based hydrogels appear to be a promising material for dentin pulp complex regeneration, and further investigation is warranted. </p>
<p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;"><br></p><p style="margin-bottom: 0px; font-style: normal; font-variant-caps: normal; font-stretch: normal; font-size: 12px; line-height: normal; font-family: Helvetica; font-size-adjust: none; font-kerning: auto; font-variant-alternates: normal; font-variant-ligatures: normal; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-position: normal; font-variant-emoji: normal; font-feature-settings: normal; font-optical-sizing: auto; font-variation-settings: normal;">Keywords: Biodentin, Dental pulp derived stem cell, Hydrogel, Levan, MTA </p>